Our latest blog on interesting facts about glycans we dig deeper into the UDP-glucuronic acid and glucuronyltransferases in the endoplasmic reticulum - which can be critical in drug metabolism.

Read at: https://research.bidmc.org/ncfg/blog/facts-about-udp-glucuronic-acid-and-glucuronosyltransferases-er

Keyhole Limpet Hemocyanin (KLH) is used to produce #antibodies and as an #immunostimulant. Interestingly 3-4% of it's weight is #carbohydrate.
In this month's interesting facts about #glycans we take a look at the unusual #glycosylation of KLH.
Read at: https://research.bidmc.org/ncfg/blog/facts-about-keyhole-limpet-hemocyanin-klh-and-its-unusual-glycosylation

Want to know where #glycans are specifically expressed in the #brain? Using smart anti-glycan reagents (SAGRs) we map the specific expression of glycans in the mouse brain. Check out poster-150 presented by Dr. Ea Tulin at the 2024 Society for Glycobiology meeting!

If you are attending the @SfG Society for Glycobiology annual meeting: find Dr. Lauren Pepi at poster 154 to discuss how the Glycomics Core at Beth Israel Deaconess Medical Center can help in #glycomics analysis and learn about a targeted MS/MS method utilizing over 200 glycan standards to study samples.

A spoonful of sugar #18

Plant receptor kinases translate and pass on chemical messages as part of many key biological processes. A new fabulous cryo-EM structure of the active complex between the kinase MIK2, the immunomodulatory peptide SCOOP and the co-receptor BAK1 shows how its binding hinges on N-glycosylation (https://doi.org/10.1038/s41477-024-01841-6)

You can rebuilt the fully glycosylated structure of the complex with https://glycoshape.org with some fancy-plant N-glycans

#glycotime FTW! #plants #science

N-glycosylation facilitates the activation of a plant cell-surface receptor | Nature Plants
https://www.nature.com/articles/s41477-024-01841-6

This month's interesting facts about glycans - we take a look at "bacterial cellulose"! Yes you heard that correct! Cellulose is found all around us in the form of clothing, wood, paper products and is usually sourced from plants. But bacteria also produce a form of it.

Read more about it at: https://research.bidmc.org/ncfg/blog/facts-about-bacterial-cellulose

For more interesting facts check out: https://research.bidmc.org/ncfg/interestingfacts/interesting-facts-about-glycans

A spoonful of sugar #17

Everyone needs a bit of sugar today, here is a treat

The oligosaccharyltransferase, or OST (EC 2.4.1.119), is a key enzyme that functionalizes nascent proteins with sugars called N-glycans

The 3D structure of this magnificent molecular machine has been solved by cryo-EM by Ana Ramirez et al. (PDB 8AGC) https://doi.org/10.1038/s41467-022-35067-x

Be like OST and plant one of the 195 N-glycans online on your protein 3D structure with https://glycoshape.org

It's great to see this #Caenorhabditis mannosidase III #glycotime manuscript out in @jbiolchem
Product of the @FWF_at project awarded to former postdoc Shi Yan showing how a specific mannosidase is necessary for the biosynthesis of a subset of complicated N-glycans.

https://www.jbc.org/article/S0021-9258(24)02446-3/fulltext

A spoonful of sugar #16

How does glycosylation affect not only structure and function of proteins, but also binding specificity?

In a new paper fresh off the #bioRxiv press https://doi.org/10.1101/2024.10.24.620132 in collaboration with Nicki Packer, Len Harrison and Ethan Goddard-Borger, we explain how glycosylation of CD52 (reconstructed with https://glycoshape.org) determines:
1) the peptide's conformation,
2) binding to the HMGB1 Box B and only then
3) to SIGLEC-10

Some exciting #glycotime to start the week!

GlycoShape is fresh off the press today on Nature Methods https://www.nature.com/articles/s41592-024-02464-7

Check out the short thread below 1/6

The Nobel Prize in Chemistry 2024 goes to David Baker (1/2) and Demis Hassabis and John Jumper (1/2) for protein structure design and prediction from sequence with AI

https://www.nobelprize.org/prizes/chemistry/2024/summary/

Congratulations to all very worthy winners from all of us!

As a reminder, just add sugars with http://glycoshape.org

A spoonful of sugar #15

Arabinose is an aldopentose (five-membered ring sugar terminating with a CHO group) commonly found in plants as part of chains O-linked to hydroxy-Pro (Hyp) residues in the protein

As an example, look below at the magnificent structure of an algal mastigoneme resolved by cryo-EM (Dai et al. doi: 10.1016/j.cell.2024.03.005)

For all your arabinose (and Galf) needs, go to https://glycoshape.org , we got you covered!

This month's interesting fact about #glyco covers rare and unusual sugars and monosaccharides like D-tagatose, D-allulose and L-sorbose.

Read more: https://research.bidmc.org/ncfg/blog/facts-about-rare-and-unusual-sugars-nature

For more interesting facts: https://research.bidmc.org/ncfg/interestingfacts/interesting-facts-about-glycans

Got questions about #glycan #microarrays, and their data analysis? We are hosting an Ask Us Anything session, where you can ask us your questions.
Note - this is not a seminar.
Join us on: September 23rd, 2024 10am-11am ET
Sign up for Zoom:
https://forms.gle/LsAYLusyMGa3Heet9
#glycotime

In this month's interesting facts we take a look at CMP-Sialic acid, a sugar donor which is key in the #sialylation of #glycans; and its synthetase.

Read more: https://research.bidmc.org/ncfg/blog/facts-about-cmp-sialic-acid-nature

For more interesting facts: https://research.bidmc.org/ncfg/interestingfacts/interesting-facts-about-glycans

René Ranzinger, member of the #MIRAGE commission, presents the MIRAGE guidelines for glycan microarray and mass spectrometry data at the GLYCOTwinning Summer School 2024, NOVA FCT, Caparica, Portugal : https://www.glycotwinning.eu/summerschool24